Entries from 2024-05-01 to 1 month

Bulk normalization summary

https://hbctraining.github.io/DGE_workshop/lessons/02_DGE_count_normalization.htmlIn summary, DESEQ2 provide median of ratio method to normalize everything capable of comparison between samples and DEG analysis, but cannot handle within sa…

Barcode method summary

We can divide all barcode lineage methods into two categories. First part is the barcode, a rondom nucleotide sequence (10-20 nt), can either be DNA (inserted by lenti or transposase to read out by NGS) or RNA (integrated on 3"UTR of repor…