Transformation 1, start with 5ul plasmid / product 2, mix it with 50ul bacteria (-80 freezer in cell culture room) (if start with finished plasmid, then 5ul aliquots should be fine) 3, Put the mixture on ice for 30 min 4, Heat shock mixtur…

Time-series IS and RNA-seq for documenting NCO culture https://docs.google.com/document/d/14Sk8b28u3MEL_Hj_jAe6HiC1uofTVvPYWlzk4iri5fo/edit?usp=sharing Tracking single cortical progenitors with NOTCH2NLB overexpression https://docs.google.…

live imaging two photon in organoids (with primates) besides lancaster paper but maybe FV3000 should be enough for that? membrane potential differenration process fesibility of measuring neuralepitheliam niche （our strong point） Symetric…