2004
Tamily A.Weissman1Patricio A.Riquelme1LidijaIvic2Alexander C.Flint2 Arnold R.Kriegstein123
We demonstrate here that calcium waves propagate through radial glial cells in the proliferative cortical ventricular zone (VZ). Radial glial calcium waves occur spontaneously and require connexin hemichannels, P2Y1 ATP receptors, and intracellular IP3-mediated calcium release. (Means such calcium waves requires neurotransimitter of ATP, and shows spontanously occuring pattern independent with extracellualr ca2+)
Atp 以及它的receptor P2Y1非常有意思因为它还参与了hyperactivity of neurons and may related with mechanical stimulation probably
Furthermore, we show that wave disruption decreases VZ proliferation during the peak of embryonic neurogenesis. (Ca2+ may regulate the neurodevelopment, or specifically speaking the symmertrical to asymmertrical pattern?) Taken together, these results demonstrate a radial glial signaling mechanism that may regulate cortical neuronal production.
The right pannel shows how they monitor five cells and record the calcium propagate pattern in a time lapse manner
A things may interesting is
1, Oscillating of Ca2+ signal detected by Fluo-3 indicator (maybe Gcamp is better?) (Probably Coupled with Notch-Hes oscillation?)
2, Temproal and spatially cluster of such propagation (as shows below)
3, can be reproduced by using a brief pulse of electrical or mechanical stimulation. 可以使用物理刺激激活calcium propagate is very intriguing, since it reinforce me the importance of mechanical factor may related with such VZ proliferation regulation.
4, The experiment they implented may can be used in our lab
