1, Marker (cool)
ventricular zone−enriched RG (vRG) that express ANXA1 and CRYAB, and outer subventricular zone−localized RG (oRG) that express HOPX. Our study identified vRG and oRG markers and molecular profiles, an essential step for understanding human neocortical progenitor development.
Somt thing cool is:
oRG also express those canonical markers of vRG, including Pax6, Sox2, that is why oRG and vRG are collectively called "SP" cells (S and P)
the coolest figure in this article
VZ and SVZ are collectively called germinal zone (becasue they consists of proliferative cells)
HOPX+SOX2+ cells (oRG) in the ventricular zone occasionally exhibited apical process anchored at the ventricular surface at 16 PCW
It is really cool because apical endfoot is used to believed one property for vRG. As the cells translate into oRG, it should lost its apicial endfoot and only maintain the basal process anchored to the pial surface.
Left pannel is their SC transcriptomic profiling methods (see below), right pannel is a real 21 PCW specimen. You can see the similarity here, but I am more interested in those cell type specific markers.
2, A new method called FRISCR
that enables targeted mRNA purification and single-cell transcriptomic profiling of fixed cells without compromising data quality compared with live cells.
A little more precise data here
(the "majority" of RG contain the sox2, not all)
Sharad Ramanathan
