Referred from Huttner 2011 Nat communication
BPs have a substantially longer G1-phase than APs, and that the previously observed G1 lengthening of neurogenic NPCs in the VZ actually reflects the increasing contribution of BPs.
(this provides a contrary viewpoint to the impression that AP cell cycle elongates as development progress)
Unexpectedly, expanding apical and basal progenitors exhibit a substantially longer S-phase than apical and basal progenitors committed to neuron production. Comparative genome-wide gene expression analysis of expanding versus committed progenitor cells revealed changes in key factors of cell-cycle regulation, DNA replication and repair and chromatin remodelling. Our findings suggest that expanding neural stem and progenitor cells invest more time during S-phase into quality control of replicated DNA than those committed to neuron production.
Tis21-GFP+ represent neurogenic division or BP production division
Tis21-GFP- is self-renewal division
Blue bars, G1-phase; red bars, S-phase; yellow bars, G2-phase; green bars, M-phase.
