One example We transcriptionally profiled a total of 18,545 mouse neocortical cells at two key times of corticogenesis using Drop-seq: 10,931 cells at embryonic day 14.5 (E14.5) from six biological replicates, and 7614 cells at birth (P0) …

Reference https://support.illumina.com/bulletins/2017/04/considerations-for-rna-seq-read-length-and-coverage-.html How many reads should I target per sample? Read depth varies depending on the goals of the RNA-Seq study. Most experiments r…

PCW and GW Defination of different chronology calibrations in human development PCW post conception week the eaiset notion GW Gestation week Basically two weeks more than PCW since ⬇ The unborn baby spends around 38 weeks in the womb, but …

Basically there are three methods for culture ware surface treatment btw, the majority of culture ware are polystyrene. Tissue-culture treated plates Polystyrene plastic must be subjected to a surface treatment to render the plastic suitab…

Near-infrared fluorescent reporters The electromagnetic radiation of a wavelength longer than visible light, but shorter than microwave radiation, having a wavelength between 700 nm and 1 mm. Light sheet microscopy Custom-built No need to …

Humans and chimpanzees share a surprising 98.8% of their DNA, while humans and gorillas share more than 96% Bonobos and chimpanzees are more closely related to humans than either of them are to the other two species of great apes: oranguta…

Human cerebral cortex expands between the late first trimester (GW10) (though suzuki sensei refers it as early as GW9) and the neonatal period (GW41), which lasting for 217 days briefly described as 7 months. Our brain progressive growth d…

In results section, please always write in this format. “to know something, I conducted something. The results were xxx. Thus, I concluded that yyy. Next remaining questions are zzz”

The Stages of Human Embryonic Development Translate neurodevelopmental time across mammaliam species

生物物理，可以帮忙测力的 Kaoru Sugimura 杉村 薫 By combining mechanical and genetic perturbations along with live imaging and Bayesian force-inference, we are addressing physical principles underlying regulation of epithelial morphogenesis…

New born neuron marker: Firstly Neurog2 and Tuj1, then NeuroD1 and Tbr2 (Tbr2 is frequently used as new born neuron marker in rodents), then NeuroD2 and Tbr1 (deep layer marker). The cascade from Hes1 (repress Ascl1 and Neurog1/2. Therefor…