Single cell RNA-seq reads number and depth

One example

We transcriptionally profiled a total of 18,545 mouse neocortical cells at two key times of corticogenesis using Drop-seq: 10,931 cells at embryonic day 14.5 (E14.5) from six biological replicates, and 7614 cells at birth (P0) from three biological replicates (Fig. 1a, Supplementary Table 1)11,17. Single-cell libraries were sequenced to a median depth of ~12,000 reads/cell, detected a median of ~2500 transcripts per cell, and represented a median of ~1600 of genes per cell (Fig. 1a, Supplementary Figures 12). This depth is on par with or exceeds similar studies11,12,14.

 

Referred from this

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